Labeling of Escherichia coli Expressed SNAP-tag ® Fusion Proteins.With proper storage at -20☌ the substrate should be stable for at least three years dry or 3 months dissolved in DMSO. Protect the substrate from light and moisture. Storage: CLIP-Biotin should be stored at -20☌ (long term) or at 4☌ in the dark (short term, less than 4 weeks).Properties & Usage Materials Required but not Supplied
The following reagents are supplied with this product: NEB # Figure 1: Structure of CLIP-Biotin (MW 569.7 g/mol) This product is related to the following categories: Biotin Labels Products, CLIP-tag™ Substrates Products, Protein Labeling Products, This product can be used in the following applications: CLIP Cell, In vivo Imaging, Protein Localization, Protein Labeling Snap Clip Reagents Supplied Reagents Supplied The labeling of fusion proteins with CLIP-Biotin is described below. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. CLIP-tag and SNAP-tag can be used for orthogonal simultaneous labeling. Although CLIP-tag is based on the same protein as SNAP-tag ®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether link. CLIP-tag substrates are derivatives of benzylcytosine (BC). CLIP-tag is a small protein based on mammalian O 6-alkylguanine-DNA-alkyltransferase (AGT). This package contains 50 nmol of CLIP-Biotin substrate, sufficient to make 10 ml of a 5 μM solution for the labeling of CLIP-tag fusion proteins in cells.ĬLIP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest.
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